An eighty-day feeding trial was conducted to evaluate the influence of different short-term fasting and re-feeding strategies including the control (fed two times daily to apparent satiation throughout the experimental period); F2R8 (2 days fasting followed by 8 days of re-feeding); F4R16 (4 days fasting followed by 16 days of re-feeding) and F8R32 (8 days fasting followed by 32 days of re-feeding) on growth and physiological responses in yellowfin seabream, Acanthopagrus latus (3.16 ± 0.2 g) fingerlings. Fish in the control and F2R8 groups had the highest and the lowest total length, respectively. Moreover, fish exposed to F4R16 and the control treatments had significantly the highest and the lowest hepatosomatic indices, respectively (P < 0.05). Fish in the F2R8 group relatively had higher catalase and glutathione-S-transferase activities than other groups (P < 0.05). Furthermore, total protease, ?-amylase and alkaline phosphatase activities in the F4R16 and F8R32 were higher than the F2R4 and control groups (P > 0.05). Overall, this study showed that the best group in terms of compensatory growth in weight and length as well as digestive enzyme activities was the F8R32; however, in this group increase the activity of the enzymes, which can be indicative of the occurrence of oxidative stress mentioned.