Numerous studies have been conducted on the potential benefits of astaxanthin for humans and animals, and the identification, production, and use of its natural sources have also been conducted; But most of the global markets are covered by its synthetic type. Astaxanthin is a secondary metabolite that is synthesized in all types of prokaryotic cells, especially microalgae. Haematococcus pluvialis is one of the most important organism that produces it with its accumulation and storage up to 4% of its total biomass. Due to the easy induction of astaxanthin synthesis in the microalgae Haematococcus pluvialis, we considered it our duty to carry out this research with the aim of molecular measurement in the qualitative expression of genes interfering in the biosynthetic pathway of the secondary compounds in question. Astaxanthin is biosynthesized in this microalgae through chemical induction and it was done by adding GABA and melatonin inducers to the culture medium containing cells and placing them under different light conditions. Finally, algae cells after passing the growth phase, in nine different conditions with three repetitions, with concentrations of zero and 0.0001 g/L of GABA and zero and 0.0001 g/L of melatonin and under LED light with three sunny colors , blue and red were treated with 8-16 sleep-wake cycle and the behaviors of the cells in production were studied by measuring the amount of absorption. In this research, variables such as temperature, light, and food consumption were kept constant and chemical stresses were applied against them, and secondary metabolite production was measured by measuring the amount of absorption and qualitatively measuring the expression of genes involved in the biochemical pathway of astaxanthin synthesis by RT-PCR technique. , was investigated. The aim of the research was to find the best stress conditions for the biosynthesis and production of the secondary metabolite of astaxanthin and fatty acids. For this purpose, a
