25 تیر 1403
ليلا كرمي

لیلا کرمی

مرتبه علمی: استادیار
نشانی: دانشکده مهندسی کشاورزی - گروه علوم و مهندسی باغبانی
تحصیلات: دکترای تخصصی / زیست شناسی علوم گیاهی
تلفن: 09173767604
دانشکده: دانشکده مهندسی کشاورزی

مشخصات پژوهش

عنوان Antimutagenic and anticancer effects of Avicennia marina leaf extract on Salmonella typhimurium TA100 bacterium and human promyelocytic leukaemia HL-60 cells
نوع پژوهش مقالات در نشریات
کلیدواژه‌ها
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مجله SCIENCEASIA
شناسه DOI
پژوهشگران لیلا کرمی (نفر اول) ، احمد مجد (نفر دوم) ، صدیقه مهرابیان (نفر سوم) ، محمد نبیونی (نفر چهارم) ، سعید ایریان (نفر پنجم) ، ماندانا صالحی (نفر ششم به بعد)

چکیده

The present study evaluated the antimutagenic and anticancer activities of A. marina leaf extract. The antimutagenic activity of ethanol and water extracts of A. marina leaf (concentration 10 mg/plate for 48 h) was evaluated using the Salmonella typhimurium TA100 strain in the Ames test with ( S9) and without (S9) metabolic activation, and its anticancer effect with concentrations of 200, 400, 450, 500, 600, and 800 µg/ml of ethanol extract for 24, 48, and 72 h on human leukaemic cell line HL-60 was assessed by MTT assay. The ethanol and water extracts of A. marina were revealed to have antimutagenic activity in the Salmonella mutation assay. The number of mutant colonies decreased in the presence of ethanol and water extract with ( S9) and without (S9) metabolic activation. The ethanol extract showed a higher antimutagenic effect than the water extract, with an inhibition rate of 71% on the mutated bacterium. The MTT cell viability test showed an induction of cytotoxicity in HL-60 cells treated with the extract with IC values of 600, 400, and 280 µg/ml after 24, 48, and 72 h, respectively, in a concentration and time-dependent manner. Flow cytometric analysis con?rmed the induction of apoptosis in the cells. Collectively, these results suggest that the Avicennia marina leaf extract contains bioactive compounds with antimutagenic and antileukaemic effects.