05 اردیبهشت 1403
ليلا كرمي

لیلا کرمی

مرتبه علمی: استادیار
نشانی: دانشکده مهندسی کشاورزی - گروه علوم و مهندسی باغبانی
تحصیلات: دکترای تخصصی / زیست شناسی علوم گیاهی
تلفن: 09173767604
دانشکده: دانشکده مهندسی کشاورزی

مشخصات پژوهش

عنوان Effect of salinity and drought stress on morphological and biochemical properties of two Iranian fenugreek (Trigonella foenum– graecum) populations
نوع پژوهش مقالات در نشریات
کلیدواژه‌ها
callus induction, drought stress,fenugreek, salinity stres, trigonelin
مجله Notulae Botanicae Horti Agrobotanici Cluj-Napoca
شناسه DOI DOI:10.15835/nbha49212038
پژوهشگران افسانه توانگر (نفر اول) ، لیلا کرمی (نفر دوم) ، محمد هدایت (نفر سوم) ، غلامرضا عبدی (نفر چهارم)

چکیده

In this study, micro propagation of two Iranian Fenugreek populations (Ardestan and Borazjan) and their morphological and biochemical responses to salinity and drought stresses in in vitro culture condition were conducted using factorial experiment in a completely randomized design in three replications. Different explant type (terminal bud, cotyledon and epicotyledon explant) were cultured in MS medium contain different concentration of plant growth regulators such as Kin (0, 0.5 and 1 mg / l) and 2,4-D (0.5, 1 and 2 mg / l). Murashige and Skoog (MS) medium supplemented with 1 mg/l kinetin and 2 mg/l 2,4-D showed the highest callus proliferation rate per explants in both papulation. The highest amount of callus volume was obtained from the explants of the terminal bud. Proliferated calli from terminal bud explant were green and yellowish, from cotyledon were yellowish to white with soft texture, and the cotyledons were greenish and compact. The results of salinity stresses with different concentrations of sodium chloride (0, 70 and 120 mM) and drought stress with polyethylene glycol (0, 5 and 10%) showed that both stresses decreased callus growth and increased total protein, proline, catalase, peroxidase and Trigonelin content in both papulation. Trigonelin measurement showed that Borazjan papulation had higher Trigonelin content in vitro than Ardestan papulation.