May 29, 2024
Leila Karami

Leila Karami

Academic Rank: Assistant professor
Address:
Degree: Ph.D in plant biology
Phone: 09173767604
Faculty: Faculty of Agricultural Engineering

Research

Title Effect of salinity and drought stress on morphological and biochemical properties of two Iranian fenugreek (Trigonella foenum– graecum) populations
Type Article
Keywords
callus induction, drought stress,fenugreek, salinity stres, trigonelin
Journal Notulae Botanicae Horti Agrobotanici Cluj-Napoca
DOI DOI:10.15835/nbha49212038
Researchers Leila Karami (Second researcher) , Mohammad Hedayat (Third researcher) , gholamreza Abdi (Fourth researcher)

Abstract

In this study, micro propagation of two Iranian Fenugreek populations (Ardestan and Borazjan) and their morphological and biochemical responses to salinity and drought stresses in in vitro culture condition were conducted using factorial experiment in a completely randomized design in three replications. Different explant type (terminal bud, cotyledon and epicotyledon explant) were cultured in MS medium contain different concentration of plant growth regulators such as Kin (0, 0.5 and 1 mg / l) and 2,4-D (0.5, 1 and 2 mg / l). Murashige and Skoog (MS) medium supplemented with 1 mg/l kinetin and 2 mg/l 2,4-D showed the highest callus proliferation rate per explants in both papulation. The highest amount of callus volume was obtained from the explants of the terminal bud. Proliferated calli from terminal bud explant were green and yellowish, from cotyledon were yellowish to white with soft texture, and the cotyledons were greenish and compact. The results of salinity stresses with different concentrations of sodium chloride (0, 70 and 120 mM) and drought stress with polyethylene glycol (0, 5 and 10%) showed that both stresses decreased callus growth and increased total protein, proline, catalase, peroxidase and Trigonelin content in both papulation. Trigonelin measurement showed that Borazjan papulation had higher Trigonelin content in vitro than Ardestan papulation.