May 29, 2024
Leila Karami

Leila Karami

Academic Rank: Assistant professor
Address:
Degree: Ph.D in plant biology
Phone: 09173767604
Faculty: Faculty of Agricultural Engineering

Research

Title
Investigation of effects of NAA and 2,4-D auxins on induction of somatic embryogenesis in desert teak (Tecomella undulata) in semi-solid culture medium
Type Thesis
Keywords
Tecomella undulata, somatic embryogenesis, Naphthalene acetic acid, 2,4-dichlorophenoxy acetic acid, semi-solid culture medium
Researchers Farzaneh Shamsi (Student) , Sasan Rastgoo (Primary advisor) , Leila Karami (Advisor)

Abstract

Tecomella undulata (Roxb.) Seem is a tree species and native to Iran that due to the importance of this plant in dry and desert areas, its high resistance in these conditions, its role in soil regeneration, creating very beautiful and attractive flowers that can be found in parks and green spaces used it. In the present study, the effects of the use of growth regulators on the induction of somatic embryogenesis of this species were investigated. It has been suggested that this plant is native to Iran and is used to treat some diseases such as white spots on the skin, spleen enlargement and syphilis. What is important about this tree is the difficulty of growing it, making its generation at risk of extinction. Since anarctic propagation through seed and cuttings is difficult to produce and seedling production is very slow, the application of tissue culture technology to grow inside the glass can be considered. The purpose of the present work is to evaluate the adjustment BA and Kin growth regulators were used for branching of the single nodules of this plant. For the callus of three growth regulators BA, 2,4-D, and NAA at different concentrations individually or in combination, and in another experiment, the microleakage of the first experiment with different concentrations of 2, 4-D, BA, Kin, NAA and IAA were used separately or in combination. Branching experiments were conducted in completely randomized design, calliogenesis experiment in factorial design in completely randomized design and microleakage testing was performed in completely randomized design. Nodule and leaf samples and MS medium were used. Disinfection of the explants was done in 96% alcohol for 45 to 60 seconds and then immersed in 0.75% sodium hypochlorite for 10 minutes. The results showed that nodal specimens treated with 3 mg / l BA, 2 and 2.5 mg / l Kin had the highest branching percentage, nodal explants with 3 mg / l BA treatments. With 1 mg / l NAA, 0.5 mg / l 2,4-D plus 3 mg / l BA, 0.75,