This study investigates the effects of short-term fasting and refeeding on the activity of digestive and antioxidant enzymes in juvenile Asian sea bass (Lates calcarifer). Short-term fasting can induce significant physiological changes in fish, affecting digestion, nutrient absorption, and the immune system. In this study, 150 juvenile sea bass were randomly divided into 6 fiberglass tanks, consisting of 1 treatment group and 1 control group, each with 3 replicates. The control group was fed twice daily to satiety throughout the experiment, while the treatment group was subjected to 8 days of fasting followed by 32 days of refeeding. Data from various experiments were processed using SPSS version 22. All data were averaged from 3 replicates with standard error, and normality was tested using the Shapiro-Wilk test, while variance homogeneity was tested using Levene’s test. A one-way analysis of variance (ANOVA) was applied to examine differences between treatments at a 5% significance level. Tukey’s multiple comparison test was used to compare means. The results showed that short-term fasting decreased the activity of digestive enzymes such as amylase, protease, and lipase. However, after refeeding, these enzymes gradually returned to their normal levels. Additionally, the activity of antioxidant enzymes such as superoxide dismutase (SOD) and catalase (CAT) increased during the fasting period, indicating an oxidative stress response in the fish, but these enzymes returned to a more balanced level after refeeding. The findings of this study suggest that juvenile Asian sea bass can regulate their enzymatic activity in response to nutritional changes, but the duration of fasting and refeeding conditions play an important role in the rapid recovery of digestive performance and reduction of oxidative stress. These findings can be applied to optimize feeding programs in aquaculture.