Ahmad Shadi

Background: Gastric cancer is a fatal disease with poor overall survival statistics worldwide, and today, increasing resistance to anticancer drugs, high levels of toxicity and side effects associated with certain chemotherapy drugs lead to an increased need for new anticancer drugs based on natural bioactive compounds. Aim: has been In this research, the antioxidant and anticancer effects of fucoidan extracted from Sargassum algae were investigated on AGS cancer cell line. Also, the effects of this fucoidan on gram positive and gram negative bacteria were studied. Methodology: Fucoidan was extracted by CaCl2 method and purified by dialysis bag. Identification and qualitative investigation of the structure of fucoidan extracted from Sargassum brown algae was done through Fourier transform infrared (FTIR) spectroscopy. Also, nuclear magnetic resonance (NMR) method was used to determine the composition and structure of extracted fucoidan. Antioxidant property was investigated by DPPH method at a concentration of 1% fucoidan and percentage of scavenging activity was calculated. The antibacterial effect was analyzed by measuring the diameter of the non-growth halo, MIC and MBC on two gram-negative and two gram-positive bacteria strains, and the measurements and data were analyzed with Prism 8 statistical software. Finally, the effect of this sulfated polysaccharide on the survival of AGS cell line was investigated by MTT assay and its effect on the migration of gastric cancer cells was investigated by wound scratch test. Conclusions: According to the findings, fucoidan showed acceptable antioxidant and anticancer effects. Also, it had a good antibacterial effect on gram-positive bacteria, but it