Background
Success in fish farming requires the production of quality feed. In aquatic diet, organic acids can be used as effective substances to improve growth performance and safety. Given the rapid progress of Asian seabass farming in the country, research should be directed towards increasing productivity in breeding this species. Therefore, in this study, the effect of organic and probiotic acids on some parameters of this species was evaluated.
Objective
The aim of this study was to investigate the combined effect of propionic acid and Bacillus subtilis on growth function, immune system, activity of digestive enzymes and antioxidants in Lates calcarifer.
Methodology
In this experiment, 180 pieces of Asian seabass with an average weight of 2.9 ± 0.11 g were used. The fish were distributed in 18 tanks with a volume of 100 liters of water and a density of 10 pieces of juvenile fish in each tank completely randomly. Experiment in 6 treatments with 3 replications Includes: 1 control treatment: (free of organic acid and probiotic) Treatment 2: 0 gram of propionic acid and 1 gram (×6/1107 cfu g-1) per kg of diet, treatment 3: 5 g of propionic acid and 0 g of probiotic per kg of diet, Tima 4: 10 g propionic acid and 0 g probiotics per kg diet, treatment 5: 5 g propionic acid and 1 g (×6/1107 cfu g-1) Probiotics per kg diet, treatment 6: 10 g propionic acid and 1 g (×6/1107 cfu g-1) Probiotics per kg of diet. Done for 5 weeks. At the end of week 5, fish morphometry was performed to determine growth performance. Liver and intestinal biopsies were performed to measure gene expression, antioxidant enzymes and digestive enzymes.
Results
Groups fed diets containing propionic and probiotic acid Bacillus subtilis had higher growth than the control group (P <0.05). The highest rate of weight gain was observed in the treatment (Pro + 10PA). Our findings in this experiment showed that the chemical composition of the bodies of fed fish or test diets were not affected by organic a