Background: Using Seaweeds or macroalgae in aquafeeds have positive impacts to enhance performance, nutrients utilization, boost immune system responses and control disease outbreaks without any negative effect on shrimp.
Aim: the aim this recent research is to examine the impact of dietary sulfated polysaccharide extracted from Sargassume spp on growth parameters, Immuno gene expression and antioxidant enzymes of the white leg shrimp (Litopenaeus vannamei).
Methodology. In this regard, five diets were formulated with sulfated polysaccharide supplementation at 0.5, 1, 2 and 4 g/kg diet were employed. A diet without sulfated polysaccharide was used as a control ach treatment was recurred 3 times a day. Shrimps were allocated into 15 circular fiberglass tanks filled with 300 L seawater (15 shrimp per tank) and each dietary treatment replicated in triplicate at 8 weeks. Shrimps were fed three times a day. Results: The results showed food conversion ratio were significantly affected by different treatments except at treatment of 4 g/kg of sulfated polysaccharide (p < 0.05). The survival rate was significantly higher in shrimp fed with dietary sulfated polysaccharide 1 g/kg compared to the control group (p < 0.05). The dietary sulfated polysaccharide had no significant effect on other growth parameters (gain body, specific growth rate, condition factor) (P > 0.05). The activity of antioxidant enzyme glutathione was increased in the hepatopancrease of shrimps fed sulfated polysaccharide supplemented diets; (P < 0.05). meanwhile, the level of lipid peroxidation (i.e., malondialdehyde concentration) reduced by increasing dietary organic acids and cinnamaldehyde level (P < 0.05).
At the end of the feeding experiment, the dietary supplementation of sulfated polysaccharide levels enhanced the expression of immune-related genes, Prophenoloxidase (ProPO), Lysozyme (Lys), Superoxide dismutase (SOD) and penaeidin 3a genes in in the hepatopancreas tissue of L. vannamei.
Conclusion: