14 آذر 1403
فرشته بيات شاه پرست

فرشته بیات شاه پرست

مرتبه علمی: استادیار
نشانی: دانشکده مهندسی کشاورزی - گروه مهندسی تولید و ژنتیک گیاهی
تحصیلات: دکترای تخصصی / اصلاح نباتات
تلفن: 07733445182
دانشکده: دانشکده مهندسی کشاورزی

مشخصات پژوهش

عنوان
غربال باکتریهای سودوموناس فلورسنت حاوی ژن فنازین یک کربوکسیلیک اسید به منظور کنترل بیولوژیک پاخوه گندم
نوع پژوهش پارسا
کلیدواژه‌ها
take-all, wheat
پژوهشگران مسعود خان احمدی (دانشجو) ، فرشته بیات شاه پرست (استاد راهنما) ، فاطمه جمالی (استاد راهنما) ، حمیدرضا نوریزدان (استاد مشاور) ، احمد قاسمی (استاد مشاور)

چکیده

Wheat take-all fungus is Gaeumannomyces graminis var. tritici of wheat in the country is important pathogens. In order to control this pathogen biology, the study of 22 different strains of Pseudomonas fluorescens strains containing phenazine 1-carboxylic acid synthase gene were used. Tracking gene by primers for genes PCA3b and PCA2a antibiotic synthesis gene and the effect on fungal strains were tested. The ability to produce antimicrobial metabolites such as siderophore production, hydrogen cyanide and antibiotics by bacteria and fungi influence on the growth of bacteria in vitro were investigated. The results showed that the bacterial strains capable of producing hydrogen cyanide, siderophore and an antibiotic were phenazine 1-carboxylic acid in vitro. In greenhouse tests of selected strains ability to control take-all on ten varieties of wheat in a completely randomized design with three replications was used. For this purpose, in a preliminary assessment, the resistance of wheat to wheat take-all fungus was evaluated based on the results, the cultivar was selected as zarin madary. Greenhouse test results showed that the bacterial strains to increase growth and reduce the pathogenicity indices were infected plants and the growth in the take-all fungus or fungus control take-all depending on different wheat varieties and strains of bacteria.