Background: Stomach cancer is the second leading cause of death from cancer. Due to the
poor prognosis of gastric cancer, the rate of early detection is low and therefore, most
patients are diagnosed with advanced and metastatic stages. So chemotherapy is used in
these people. Cisplatin plays a vital role in the treatment of undiagnosed cancer micro-foci
and free cancer cells, but it faces limitations such as non-specific effects and multi-drug
resistance. One of the primary solutions for this challenge is the combination therapy with
magnetic nanoparticles. Combination therapy can help overcome drug resistance. This
treatment involves the simultaneous transfer of nucleic acids with chemotherapy drugs that
change the resistance mechanism in the tumor. The use of magnetic nanoparticles in targeted
delivery of cisplatin and genes minimizes side effects. Therefore, it can be an effective
treatment.
Aim : Investigating the effect of codelivery of OCT4 shDNA and cisplatin by magnetic
nanoparticles on the growth, death and migration of AGS cells and the level of OCT4 gene
expression. Also investigating the role of suppressing the expression of this gene on
cisplatin resistance.
Methodology: Fe3O4 nanoparticles were synthesized by co-precipitation method.
nanoparticles were coated with PEI, then cisplatin loaded and in the last stage nanocomplex
were coated with dextran. XRD, FT-IR, SEM and VSM analyzes are used for the
characterization of nanoparticles. After confirming the properties of the nanoparticle, OCT4
shDNA was loaded onto the nanoparticle, and the anticancer properties of the nanoparticle
were evaluated with Cellular uptake, MTT, Scratch Assay, Apoptosis assay, and Real Time
PCR tests.
Findings: The results showed that the nanocomplex enters the cancer cells in a short period
of time. The nanocomplex carrying cisplatin leads to decrease in survival and migration of
AGS cell line and increases apoptosis. Also, this nanocomplex increases the expression of
the apop