لیلا کرمی

In Bushehr province of Iran, Avicennia marina trees have grown in Bordekhoon (Mond Protected Area) and Assaluyeh (Marine National Park of Nayband). Contrary to Bordekhoon, Assaluyeh is aAssaluyeh pollen extracts, respectively, and mice serum samples were used for immunoblotting. Pollen characteristics were studied using light and scanning electron microscopes. SDS-PAGE showed some differences between pollen protein pro?les of two regions. Immunoblotting assay detected that pollens have two allergenic bands and the protein band at 100 KD is the common allergenic protein in two regions. Light microscopy revealed that the development of anther wall was basic type and some abnormalities were observed in microspores and pollens of Assaluyeh. Scanning electron microscopy studies showed that the apertures in considerable numbers of Assaluyeh pollens were closed. The comparison of elemental composition of pollen tectum between two regions showed that pollens of Assaluyeh have accu- mulated Cu on their tectum. Results obtained indicated that environmental pollution can affect protein pro?le, allergenic bands, structure and elemental composition of tectum of A. marina pollens. petrochemical region with environmental pollution. This study was aimed to studying protein pro?les, allergenic bands, ontogeny, structure and elemental composition of tectum of A. marina pollens in Assaluyeh and Bordekhoon. Pollens were collected from two regions and extracted in PBS, and protein pro?les of pollens were determined by sulfate–polyacrylamide gel electrophoresis (SDS-PAGE). As an experimental model, 20 female 6–8-week-old Balb/C mice were divided into two groups. The mice of ?rst and second groups were sensitized by Bordekhoon and