محمد هدایت

Microbial contamination under in vitro tissue culture is considered as one of the most important limiting factors for micropropagation. However, due to the unique properties of palm tree under long -time tissue culture period, identification of media contaminants is important for the application of the best method of control. In this study five different genera of fungi were identified as a source of pollution in palm tissue culture. It was revealed that the fungal infection in palm tissue culture media was caused byAspergillus spp. (43%),Rhizoctonia spp. (21%), Fusarium spp. and Penicillium spp. (each with 14 %), respectively. Caladosporiom spp. with 8% abundance caused the least infection in the tissue culture. The fungi identified in this study decreased the success of tissue culture significantly.