محمد هدایت

Ber (Ziziphus), while the pharmaceutical and cosmetic industry is an important medicinal plant of tropical fruits are abundant properties. Because of the limitations in traditional breeding methods (crossing and selection) and the other ber features a high heterozygosity, use of new technologies as complementary Molecular traditional correction techniques to improve the economic characteristics is required. Study on somatic embryogenesis in many scientific aspects and applications. Somatic embryogenesis in comparison with other methods of asexual reproduction is superior because it allows mass propagation of plants using bioreactor provides. This study, in order to obtain optimal protocols for direct and indirect embryogenesis from different explants with Bengali (Ziziphus mauritiana Lamk.). In this study, the basal medium MS, Tuesday explants including leaves, buds, terminal and axillary bud, four hormone-containing 2,4-D (1 level), BA (8 levels), IAA (2 levels), NAA (2 levels) and sucrose (2 levels ) was used. The results indicated the interaction effects of BA, IAA and medium containing 6 mgl-1 BAwith 0.03 mgl-1 IAA best combination hormonal treatment for induction of callus in lateral bud explants. Medium containing 30 gl-1 of sucrose, with 0.0 l mgl-1 2,4-D and 3 mgl-1BA causing the embryo had formed calluses. On MS medium containing 0.1 mgl-1 BA 0.1 mgl-1 NAA interaction leads to the formation of somatic embryos that formed embryos were grown in the same medium.