The effect of zeolite and nano-zeolite were examined on prolineconcentration in biological plant activity under salinity stress. This study was done in a complete randomized basic block design in the split split plat with three factors including: zeolite, salinity and basil cultivars. Treatments consisted of four level of zeolite 0, 5,10 and 15% by volume and three levels of nano-zeolite 1, 2.5 and 5% were considered. Salinity treatment was donein three level of 0, 30 and 60 mMin two cultivars of basil green and purple. Results showed that rising in salinity increased proline in leaves. Also, nano-zeolite in comparison to zeolite has a greater enhancement on proline green basin reaction in proline production was greater than purple basil. Overally, three reciprocal effects, demonstrated that nano-zeolite in 5% level accompanying with 60 mM salinity tend to the gretest degree of proline in green basil.