The purpose of this study was to investigate the effect of natural zeolite and zeolite as the part of substrate on the yield of two varieties of basil (Ocimum basilicum) under salinity stress. The split-plot experiment was conducted in a completely randomized design (CRD) with three factors of salinity, zeolite and basil cultivar. Salt stress was applied using NaCl in three concentrations of 0, 30 and 60 mM. Zeolite was used at different levels of 0, 5, 10 and 15% and nano zeolite at 0, 1, 2.5 and 5% (V/V). The results showed that the highest yield was belonged to green basil cultivar in 5% nano zeolite treatment under salt stress conditions (52.5 g), which had a significant difference with other treatments at 5% level. In general, the addition of zeolite and nano zeolite into substrate reduced the negative effect of salinity.