December 22, 2024
Mahmood Niad

Mahmood Niad

Academic Rank: Associate professor
Address:
Degree: Ph.D in chemistry
Phone: 07136333174
Faculty: Faculty of Nano and Biotechnology

Research

Title
Investigating the effect of magnetic nanoparticle which carries cisplatin and short hairpin RNA encoding gene against MALAT1 on gastric cancer cell line
Type Thesis
Keywords
سرطان معده، سيس پالتين، رده سلولي AGS، ژن 1MALAT، نانوذره ي مغناطيسي فريت كبالت، RNA كوتاه سنجاق سري
Researchers fatemeh daryani (Student) , Amirhossein Ahmadi (Primary advisor) , Hossein Nikmanesh (Primary advisor) , Mahmood Niad (Advisor) , Mohsen Nowrouzi (Advisor)

Abstract

Background: Stomach cancer is a fatal disease that is reported as the fifth most common cancer and the fourth cause of death worldwide in 2020. The limited effectiveness and side effects of current treatments, the resistance of stomach cancer cells to drugs and chemotherapy have caused new treatment strategies, especially the use of nanotechnology and nanoparticles, to be considered. Among the anti-tumor nanotherapies, one of the effective strategies is the use of magnetic nanoparticles to improve targeting, gene silencing and drug delivery, which provides a positive and powerful performance to solve the problems of conventional cancer chemotherapy. For this purpose, in the current research, the magnetic nanodrug delivery system, including two therapeutic components attached to cobalt ferrite nanoparticle and its polymer coating, has been selected Aim: In this study, the use of cobalt ferrite magnetic nanoparticles (CoFe2O4) as targeted carriers in the controlled delivery of cisplatin and shRNA MALAT1 in AGS cancer cell line has been investigated. Also, the toxicity induced by this targeted vector on the growth, migration and death rate of gastric cancer cells was studied, and the effect of cobalt ferrite nanocomplex on the expression level of MALAT1 LncRNA related to gastric cancer drug resistance was also investigated Methodology: Pure cobalt ferrite nanoparticle was made by hydrothermal method. Using a multi-step process, it was covered with polyethylene-imine and chitosan, carboxylated cis-platinum drug and MALAT1 shDNA gene were loaded into it. The morphology, structural and magnetic properties of nanoparticles were investigated by FT-IR, XRD, VSM, EDAX, FESEM and HRTEM methods. Then, in order to investigate the biological effects of the prepared nanocomposite, MTT, Scratch, Apoptosis and RT-PCR bioassays were performed Findings: The results of this research showed that the magnetic nanocomposite carrying the desired drug and gene is able to enter the nucleus o