Background and, aim: Due to the effectiveness of biosurfactant and, its biocompatibility,
the need for biosurfactants is constantly increasing. In contrast, chemical surfactants are toxic
and, non-degradable compounds; In this regard, in the present study, after extracting the
biosurfactant of Aneurinibacillus sp. and, Salinicoccus sp. bacteria, their antibacterial, antibiofilm,
antioxidant and, anti-cancer properties were investigated. Considering that the
biosurfactant produced by symbiotic microorganisms is more effective and, less research has
been done on marine microorganisms, in this research we worked on biosurfactants produced
by symbiotic bacteria native to the Persian Gulf.
Materials and, methods: Aneurinibacillus sp. and, Salinicoccus sp. bacteria; Among the 9
symbiotic bacteria with Haliclona simulans sponge that were isolated in previous researches;
were selected as the most efficient bacteria in terms of biosurfactant production. After
extracting biosurfactant from them, FTIR and, HNMR instrumental analyzes were used in
order to confirm, identify and, determine some characteristics of biosurfactants. The effect of
biosurfactants on pathogenic bacteria Staphylococcus aureus ATCC 259 and, Escherichia coli
ATCC 25922 was investigated. The effect of biosurfactants on the biofilm of Staphylococcus
aureus ATCC 259 was evaluated. The antioxidant properties of both biosurfactants were
investigated by the DPPH radical method. The effect of biosurfactants on the viability of cell
line 5637 after 48 hours was evaluated by MTT method. The effect of Salinicoccus sp.
biosurfactant on the cell cycle and, apoptosis of the SW480 cell line at a concentration of 100
μg/ml after 48 hours was investigated using flow cytometry.
Results: The results of this research showed that both biosurfactants were glycolipid and,
aliphatic. Biosurfactant produced by Salinicoccus sp. bacteria had antibacterial properties on
Staphylococcus aureus ATCC 259 and, Escherichia coli ATCC 25922