ساسان راستگو

Desert teak from Bignoniaceae family is of very beautiful flowering tree species of the southern regions of Iran that due to lack of seed setting and seed reproduction, being difficult-to-root and unavailability of an efficient vegetative propagation method, and also declining its stands because of numerous environmental and human factors, is under threat of extinction. Tissue culture propagation for this species using nodal, internodal and callus explants has not yielded much success so far. In this research, stamen and pistil explants of the plant was used in culture media containing different combinations of growth regulators i.e benzyl adenine, indole-3-acetic acid and gibberellic acid in order to be investigated their regeneration response and evaluated the possibility of plantlet production through this method. The results showed that ovary tissue has high capability for growth and development in the direction of organ and embryo regeneration. Also, stigma tissue by producing callus in most of the experimental treatments, exhibited fairly good potential for indirect (callus-mediated) regeneration. Therefore, optimization of culture medium, and type, concentration and combination of different plant growth regulators can result in promising results for successful in vitro propagation of this valuable plant species.