|
چکیده
|
Fishes of family Sillaginidae -order Perciformes- are considered as suitable candidate for aquaculture in estuaries and coastal shallow areas. COI Gene sequencing was used to achieve basic genetic information on Sillaginids in the area. In the present study, ten specimens were collected from each of Bushehr and Hormozgan provinces, and then were morphologicall and biometrical analysis was performed. Meristic counts and morphometric traits including body length, fin length, eye diameter and head length were determined by Imagej software and analysed usind Excel and SPSS software's. In order to study the phylogenetic relations of Sillaginidae family, COI sequencing methodology was used. After sequencing, data were analyzed using Chromas ver 2.33, BioEdit ver 7.0 , MEGA ver 5 and DNAsp ver 5.10. As a result of investigation of sequences and phylogenetic relationships of the samples and fulfillment of BLAST, they divided into 3 groups (potential species) and genetic statistics were calculated separately for these groups. Haplotype diversity in groups 1, 2 and 3 were high and caclculated 1, 1, 0.95 respectively. The average GC in groups 1, 2 and 3 Was obtained 48.1, 46.75 and 46.34 respectively. Based on the results of the present study, apparently group 1compared to other groups in the waters of the persian golf province is more ancient .The genetic distance of 0.225, 0.226 and 0.20 amongst three groups were in the range of species in the same genus. The results showes high genetic differentiation, which enhances the possibility of different Sillaginids species or subspecies existance in Persian Gulf. It is recommended, however, other genetic markers and microscopic examination also be used to complete this study.
|