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Abstract
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A protocol for direct embryogenesis induction without an intervening
callus production was developed in valerian (Valeriana officinalis L.) using the
leaf segments. Direct somatic embryogenesis was induced using by halfstrength
MS medium supplemented with 2,4-D (0.5 mg·l-1), glutamic acid (100
mg·l-1), 4% sucrose and 8 g·l-1 agar. The embryo germination and plantlet formation
were enhanced on MS medium supplemented with NAA (0.1 mg·l-1) and
Kin (2 mg·l-1). Regenerated plants with well-developed root and shoot systems
were successfully (72%) transferred to the greenhouse.
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