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Abstract
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An experiment was carried out to assess the potential use and applicability of shoot-tip micrografting technique for the development of virus free and plant in "Washington Navel" orange on to ‘Mexican’ lime and soure orange seedling as rootstocks. Also, the effect of different MS culture media along with different combinations of NAA and BA on proliferation rate of Washington Navel orange, grapefruit, Citrus limon and Limequat were evaluated. In another experiment the effect of different concentration of sucrose on seed germination of rootstocks were conducted. According to the species the optimum results were varied. Optimum proliferation rate for Navel orange and grapefruit were obtained in MS medium contain 2 mg/l- BA 0/5 mg/l- NAA, while, MS medium contain 1mg/lit BA 0/5 mg/lit NAA and 0/5 mg/l- BA 1 mg/l- NAA combination showed high proliferation rate for Citrus limon and Limequat respectively. Using Washigton Navel orang shoot-tip as scion, highest record of successful grafting (77/8%) obtained when soure orange seedling were used as rootstock at medium containing 60 mg/l- sucrose. Overall, these results indicated that success rate of micrografting affected by the kind of rootstock and sucrose concentration in medium.
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