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Title
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Biomimetic synthesis and characterization of cobalt nanoparticles
using apoferritin, and investigation of direct electron transfer
of Co(NPs)–ferritin at modified glassy carbon electrode to design
a novel nanobiosensor
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Type
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Article
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Keywords
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Nanoparticles Biosensor
Direct electron transfer
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Abstract
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Oxyhydroxy cobalt CoO(OH) nanoparticles
(Co-NPs) were prepared in horse spleen apoferritin (HsAFr)
cavity. Transmission electron microscopy revealed the par-
ticle size was 5.5–6 nm. Mineralization effect on HsAFr was
investigated by fluorescence and far-UV circular dichroism
(far-UV CD) spectroscopies. The far-UV CD experiments
indicated an increase in the a-helical content after mineral-
ization. Intrinsic fluorescence data showed that mineraliza-
tion acts as a quencher of HsAFr. For the first time, direct
electron transfer between Co(NPs)–HsAFr and a glassy
carbon electrode in the thin film of dihexadecylphosphate
(DHP) was investigated by cyclic voltammetry (CV) to
design a biosensor. The anionic surfactant DHP was used to
achieve direct electron-transfer between Co(NPs)–HsAFr
molecules and the GC electrode surface. CV result showed
clearly a pair of well-defined and quasi-reversible redox
peaks arise from Co(NPs)–HsAFr embedded in DHP film.
This novel biosensor can be used in medical and industrial
fields to detect different analytes.
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Researchers
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Soheila Kashanian (First researcher) , Fereshteh Abasi Tarighat (Second researcher) , Ronak Rafipour (Third researcher) , Maryam Abbasi Tarighat (Fourth researcher)
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