مشخصات پژوهش

Oxyhydroxy cobalt CoO(OH) nanoparticles (Co-NPs) were prepared in horse spleen apoferritin (HsAFr) cavity. Transmission electron microscopy revealed the par- ticle size was 5.5–6 nm. Mineralization effect on HsAFr was investigated by fluorescence and far-UV circular dichroism (far-UV CD) spectroscopies. The far-UV CD experiments indicated an increase in the a-helical content after mineral- ization. Intrinsic fluorescence data showed that mineraliza- tion acts as a quencher of HsAFr. For the first time, direct electron transfer between Co(NPs)–HsAFr and a glassy carbon electrode in the thin film of dihexadecylphosphate (DHP) was investigated by cyclic voltammetry (CV) to design a biosensor. The anionic surfactant DHP was used to achieve direct electron-transfer between Co(NPs)–HsAFr molecules and the GC electrode surface. CV result showed clearly a pair of well-defined and quasi-reversible redox peaks arise from Co(NPs)–HsAFr embedded in DHP film. This novel biosensor can be used in medical and industrial fields to detect different analytes.