December 22, 2024
Mahmood Niad

Mahmood Niad

Academic Rank: Associate professor
Address:
Degree: Ph.D in chemistry
Phone: 07136333174
Faculty: Faculty of Nano and Biotechnology

Research

Title
Investigating the effect of cisplatin and OCT4 shDNA co-delivery using magnetic nanoparticles on gastric cancer
Type Thesis
Keywords
سرطان معده، درمان هدفمند، نانوذره مغناطيسي، درمان تركيبي، سيس پلاتين، ژن OCT4
Researchers fatemeh azami (Student) , Amirhossein Ahmadi (Primary advisor) , Hossein Nikmanesh (Primary advisor) , ُAhmad qasemei (Advisor) , Mahmood Niad (Advisor)

Abstract

Background: Stomach cancer is the second leading cause of death from cancer. Due to the poor prognosis of gastric cancer, the rate of early detection is low and therefore, most patients are diagnosed with advanced and metastatic stages. So chemotherapy is used in these people. Cisplatin plays a vital role in the treatment of undiagnosed cancer micro-foci and free cancer cells, but it faces limitations such as non-specific effects and multi-drug resistance. One of the primary solutions for this challenge is the combination therapy with magnetic nanoparticles. Combination therapy can help overcome drug resistance. This treatment involves the simultaneous transfer of nucleic acids with chemotherapy drugs that change the resistance mechanism in the tumor. The use of magnetic nanoparticles in targeted delivery of cisplatin and genes minimizes side effects. Therefore, it can be an effective treatment. Aim : Investigating the effect of codelivery of OCT4 shDNA and cisplatin by magnetic nanoparticles on the growth, death and migration of AGS cells and the level of OCT4 gene expression. Also investigating the role of suppressing the expression of this gene on cisplatin resistance. Methodology: Fe3O4 nanoparticles were synthesized by co-precipitation method. nanoparticles were coated with PEI, then cisplatin loaded and in the last stage nanocomplex were coated with dextran. XRD, FT-IR, SEM and VSM analyzes are used for the characterization of nanoparticles. After confirming the properties of the nanoparticle, OCT4 shDNA was loaded onto the nanoparticle, and the anticancer properties of the nanoparticle were evaluated with Cellular uptake, MTT, Scratch Assay, Apoptosis assay, and Real Time PCR tests. Findings: The results showed that the nanocomplex enters the cancer cells in a short period of time. The nanocomplex carrying cisplatin leads to decrease in survival and migration of AGS cell line and increases apoptosis. Also, this nanocomplex increases the expression of the apop