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کلیدواژهها
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Ag@Ag2O NCs; Leaf shape core-shell nanocomposite; Host-guest recognition; L-
Tryptophan; L-Tyrosine; PLS
1. Introduction
Amino acids are the building blocks of proteins. There are approximately 20 various amino acids
that combine in various ways. Tryptophan (Try) and tyrosine (Tyr) are the two aromatic amino
acids in proteins. These amino acids are involved in numerous biological processes and are
critically crucial in human life activities. The synthesis of hormones as well as the transmission of
nerve signals and the squelching of reactive oxygen species in the human brain, are all
accomplished by these amino acids and their secondary metabolites. Tyr and Try, compared to
other amino acids, are particularly significant to the brain's nervous system and play a role in
maintaining the equilibrium of the neurological system.
Tryptophan has been identified as particularly relevant in the context of pathological diseases like
depression, according to research on the molecule's activity. F
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چکیده
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In the present study, a simple, innovative, and economically beneficial method has been proposed for the synthesis of Ag@Ag2O core-shell nanocomposites using Acanthophora muscoides algae extract. The host-guest recognition of targets was performed by modification of the Ag@Ag2O surface using β-CD. The Ag@Ag2O- β-CD NCs were used as a colorimetric sensor to determine L-Tryptophan and L-Tyrosine using a partial least square (PLS) approach. A crystalline hybrid structure of Ag core and an Ag2O shell was confirmed by XRD, FTIR, TEM and AFM research. Also, DLS analysis and surface zeta potential spectra illustrated the aggregated nature of nanocomposites in the presence of analytes. The literature review shows that the colorimetric simultaneous determination of L-Tryptophan (L-Try) and L-Tyrosine (L-Tyr) has not been reported. The Ag@Ag2O- β-CD sensor exhibited outstanding sensing capability in a broad linear range of 2.0 –200 μM for both amino acids and low detection limit of 0.32 and 0.51 μM, for L-Try and L-Tyr, respectively. The good sensitivity and excellent selectivity regarding possible interfering species, originated from the synergistic effect of host-guest recognition in combination with colorimetric sensing. Additionally, determination of analytes in various pharmaceutical, supplement and urine samples, approved the practical applicability of the constructed sensor. The computed results confirmed that colorimetric sensing in conjunction with a PLS technique was appropriate for the precise and accurate simultaneous determination of target amino acids in complex mixtures with RMSEP less than 2.5% and recovery in the range of 103–108% with R.S.D. values less than 3%.
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