مشخصات پژوهش

Nicotinamide adenine dinucleotide (NADH) is the most important directing coenzyme in redox reactions of cell metabolism, which is set to become a vital factor in diagnosing diseases such as Alzheimer’s, cancer, and Par kinson’s. The presented paper takes a look at an approach based on preparing an innovative zeolite-based sensing platform for determination of NADH in human plasma samples, the electrochemical modifier agents are ZSM-5 (Zeolite Socony Mobil–5) and Cu/ZSM-5. A striking feature of zeolites is their porous structure and the presence of extra framework cations, which enables using zeolites as effective modifier agents. In an attempt to study the morphology of synthesized modifier agents, various approaches including XRD, FTIR, EDX, SEM, HR-TEM, N 2 physisorption, and XPS were applied. To investigate the electrochemical feasibility and determination purposes of NADH in biological samples by applying the Cu/ZSM-5-based modified gold electrode, cyclic voltammetry (CV) and Differential pulse voltammetry (DPV) have been used respectively. The analytical features, including the detection limit and linear range, were obtained 0.52 μM and 0.99 to 29.12 μM respectively. These results mean a suitable linear range and a low detection limit, as well as having a 93.23 % recovery in investigation of the accuracy in the human plasma sample, confirming the good performance of the provided sensing assay in real samples. Our method significantly exhibits stable responses with high repeatability and reproducibility. Addi tionally, the interference study for NADH determination in the presence of tryptophan, L-cysteine and glucose species was effectively investigated and the appropriate performance of the developed sensing assay was confirmed.